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Article

Peptide mapping using thermospray LC/MS detection: rapid identification of hemoglobin variants.

Peptide research - 1 Jan 2000

Stachowiak K, Dyckes D F

Abstract excerpt

A rapid and easily interpreted method for peptide mapping is demonstrated with hemoglobin A and three variants. Digests of the globin chains are generated in columns containing immobilized trypsin. The resulting protein fragments are resolved by reverse phase HPLC and then analyzed by thermospray mass spectrometry. The entire process is carried out on-line. The result is a chromatographic trace which is two...

Topics

Amino Acid Sequence
Carboxypeptidases
Chromatography, High Pressure Liquid
Genetic Variation
Globins
Hemoglobin A
Humans
Macromolecular Substances
Mass Spectrometry
Molecular Sequence Data
Peptide Fragments

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Peptide Mapping

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PMID: 2520764

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2520764-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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