Article
Identification and characterization by high-performance liquid chromatography/electrospray ionization mass spectrometry of a new variant hemoglobin, Mataro [beta134(H12) Val > Ala.
Journal of mass spectrometry : JMS - 1 Aug 2001
Motos Gallardo A, Hernández J A, Hernández J M, Rovira J M, Fluvià L, Bosch A, Pastor M C
Abstract excerpt
This work illustrates the practical use of combined microbore reversed-phase high-performance liquid chromatography (RP-HPLC) with electrospray ionization mass spectrometry (ESI-MS) in protein identification. The approach consisted of the detection of the abnormal beta-globin chain by ESI-MS analysis of mixtures of intact globins, which simultaneously provided their molecular masses. Separation of the polypeptide...
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