Article

In silico based Collision Energy optimization for targeted MRM assay of Hemoglobin variants using Skyline

2025-06-06

Abstract excerpt

<title>Abstract</title> <p>Abstract: Hemoglobinopathies are among the most prevalent monogenic disorders worldwide, resulting from structural mutations or imbalances in globin chain synthesis. Accurate detection of hemoglobin variants such as HbS, |Hb Q-India, Hb A2-India and HbE is critical for diagnosis and management. Traditional methods like electrophoresis and HPLC can lack specificity or resolution. Mass sp...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0055f556-2d7d-579f-9990-850b96fcd1a2
DOI: 10.21203/rs.3.rs-6823147/v1

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

In silico based Collision Energy optimization for targeted MRM assay of Hemoglobin variants using SkylineDOI 10.21203/rs.3.rs-6823147/v1Read

Identification of hemoglobin variants by top-down mass spectrometry using selected diagnostic product ions.Semantic proximity Read

Optimization and Identification of Single Mutation in Hemoglobin Variants with 2,2,2 Trifluoroethanol Modified Digestion Method and Nano-LC Coupled MALDI MS/MS.Semantic proximity Read

Hemoglobin variant analysis of whole blood and dried blood spots by MS.Semantic proximity Read

Automated identification of amino acid sequence variations in proteins by HPLC/microspray tandem mass spectrometry.Semantic proximity Read

Increased EThcD efficiency on the hybrid Orbitrap Excedion Pro Mass Analyzer extends the depth in identification and sequence coverage of HLA class I immunopeptidomesSemantic proximity Read

Picky – a simple online method designer for targeted proteomicsSemantic proximity Read

LC-MS/MS-PRM Quantification of IgG glycoforms using stable isotope labeled IgG1 Fc glycopeptide standardSemantic proximity Read

MALDI-TOF mass spectrometry.Semantic proximity Read

Mass spectrometry: a tool for enhanced detection of hemoglobin variants.Semantic proximity Read

Rapid and specific detection of clinically significant haemoglobinopathies using electrospray mass spectrometry-mass spectrometry.Semantic proximity Read

Structural characterization of hemoglobin variants using capillary electrophoresis and fast atom bombardment mass spectrometry.Semantic proximity Read

Preanalytical Strategies for Native Mass Spectrometry Analysis of Protein Modifications, Complexes, and Higher-Order Structures<b></b>Semantic proximity Read