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Article

Investigating the binding specificity of U1A-RNA by computational mutagenesis.

Journal of molecular biology - 7 Jan 2000

Reyes C M, Kollman P A

Abstract excerpt

The mammalian spliceosomal protein U1A binds a hairpin RNA with picomolar affinity. To examine the origin of this binding specificity, we carried out computational mutagenesis on protein and RNA residues in the U1A-RNA binding interface. Our computational mutagenesis methods calculate the relative binding affinity between mutant and wild-type as the sum of molecular mechanical energies and solvation free energies...

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Source

Identifiers

PMID: 10623503
DOI: 10.1006/jmbi.1999.3319

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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