Article
The role of positively charged amino acids and electrostatic interactions in the complex of U1A protein and U1 hairpin II RNA.
Nucleic acids research - 1 Jan 2006
Law Michael J, Linde Michael E, Chambers Eric J, Oubridge Chris, Katsamba Phinikoula S, Nilsson Lennart, Haworth Ian S, Laird-Offringa Ite A
Abstract excerpt
Previous kinetic investigations of the N-terminal RNA recognition motif (RRM) domain of spliceosomal protein U1A, interacting with its RNA target U1 hairpin II, provided experimental evidence for a 'lure and lock' model of binding in which electrostatic interactions first guide the RNA to the protein, and close range interactions then lock the two molecules together. To further investigate the 'lure' step, here...
Topics
- Amino Acid Sequence
- Amino Acids, Basic
- Computer Simulation
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Binding
- RNA, Small Nuclear
- RNA-Binding Proteins