Article
Native conformational dynamics of the spliceosomal U1A protein.
The journal of physical chemistry. B - 5 Mar 2015
Guzman Irisbel, Ghaemi Zhaleh, Baranger Anne, Luthey-Schulten Zaida, Gruebele Martin
Abstract excerpt
The complex of spliceosomal U1A protein and its cognate SL2 RNA is a prototype system for protein-RNA binding studies. A major question is whether U1A protein alone is capable of undergoing conformational dynamics similar to structural rearrangements upon RNA binding. Using a fast temperature jump and tryptophan fluorescence detection, we uncover a ∼20 μs conformational transition for the Lys22Gln/Phe56Trp-only...
Topics
- Amino Acid Sequence
- Molecular Dynamics Simulation
- Molecular Sequence Data
- Mutation
- Protein Folding
- Protein Structure, Secondary
- Ribonucleoprotein, U1 Small Nuclear
- Temperature