Article
The coiled coil dimerization element of the yeast transcriptional activator Hap1, a Gal4 family member, is dispensable for DNA binding but differentially affects transcriptional activation.
The Journal of biological chemistry - 7 Jan 2000
Hach A, Hon T, Zhang L
Abstract excerpt
The heme activator protein Hap1 is a member of the yeast Gal4 family, which consists of transcription factors with a conserved Zn(2)Cys(6) cluster that recognizes a CGG triplet. Many members of the Gal4 family contain a coiled coil dimerization element and bind symmetrically to DNA as homodimers. However, Hap1 possesses two unique properties. First, Hap1 binds asymmetrically to a direct repeat of two CGG...
Topics
- Binding Sites
- Cytochrome c Group
- Cytochromes c
- DNA-Binding Proteins
- Dimerization
- Fungal Proteins
- Multigene Family
- Mutation
- Protein Binding
- Protein Structure, Secondary
- Saccharomyces cerevisiae