HAP1 positive control mutants specific for one of two binding sites.

doi:10.1101/gad.6.10.2001

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Article

HAP1 positive control mutants specific for one of two binding sites.

Genes & development - 1 Oct 1992

Turcotte B, Guarente L

Abstract excerpt

The expression of the yeast CYC1 and CYC7 genes is controlled by the HAP1 activator. A GAL4-like zinc finger (residues 1-148) specifies binding to the dissimilar sites UAS1 (of CYC1) and CYC7, and an acidic domain (residues 1307-1483) is essential for activation of transcription. To analyze how HAP1 binds to UAS1 and CYC7, we performed saturation mutagenesis of the DNA-binding domain and recovered mutants with...

Topics

Amino Acid Sequence
Base Sequence
Binding Sites
Cytochrome c Group
DNA, Fungal
DNA-Binding Proteins
Fungal Proteins
Genes, Fungal
Molecular Sequence Data
Mutagenesis
Mutation
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Trans-Activators

Transcription Factors

Zinc Fingers

beta-Galactosidase

Source

Open in PubMed

Identifiers

PMID: 1327959
DOI: 10.1101/gad.6.10.2001

Genes

HAP1
CYC1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1327959-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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