Article
Use of real-time PCR and fluorimetry to detect lamivudine resistance-associated mutations in hepatitis B virus.
Antimicrobial agents and chemotherapy - 1 Jul 1999
Cane P A, Cook P, Ratcliffe D, Mutimer D, Pillay D
Abstract excerpt
Very rapid amplification of DNA by PCR in small volumes can be continuously monitored by the detection of the binding of probes with a rapid cycler with built-in fluorometric detection. Primers were designed to amplify approximately 100 bp of the polymerase gene of hepatitis B virus (HBV) spanning codon 550, where mutations associated with resistance to lamivudine invariably occur. Four hybridization probes were...
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