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Article

Hepatitis C genotype determination by melting curve analysis with a single set of fluorescence resonance energy transfer probes.

Clinical chemistry - 1 Dec 2002

Bullock Grant C, Bruns David E, Haverstick Doris M

Abstract excerpt

BACKGROUND: The genotype of hepatitis C virus (HCV) is a predictor of antiviral therapeutic response. We describe an approach for HCV genotype determination by real-time PCR and melting curve analysis. METHODS: After automated nucleic acid extraction, we used reverse transcription-PCR in a block cycler to amplify nucleotides 6-329 of the 5'-untranslated region of HCV. The product was further amplified by...

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PMID: 12446470

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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