Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Rapid detection of hepatitis B virus mutations using real-time PCR and melting curve analysis.

Hepatology (Baltimore, Md.) - 1 Sept 2002

Zhang Manna, Gong Yuewen, Osiowy Carla, Minuk Gerald Y

Abstract excerpt

Current methods of detecting hepatitis B virus (HBV) mutations are time consuming, labor intensive, and not suitable for screening large numbers of samples. In the present study, we documented the advantages of a system that exploits differences in thermal stability between perfect match and mismatch hybrids, and thereby distinguishes between wild-type and mutants. Hybridization probes were designed complementary...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Codon, Terminator
Share to Hepatitis B
Share to Hepatitis B Surface Antigens

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hepatitis B virus

Share to Nucleic Acid Hybridization

Share to Oligonucleotide Probes

Share to Promoter Regions, Genetic

Share to Sensitivity and Specificity

Source

Identifiers

PMID: 12198666
DOI: 10.1053/jhep.2002.35346

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Rapid detection of hepatitis B virus mutations using real-time PCR and melting curve analysis.PMID 12198666Read ↗

Hepatitis B virus quantification and detection of YMDD mutants in a single reaction by real-time PCR and annealing curve analysis.Semantic proximity Read ↗

Quantification and genotyping of hepatitis B virus in a single reaction by real-time PCR and melting curve analysis.Semantic proximity Read ↗

Hepatitis C genotype determination by melting curve analysis with a single set of fluorescence resonance energy transfer probes.Semantic proximity Read ↗

Use of real-time PCR and fluorimetry to detect lamivudine resistance-associated mutations in hepatitis B virus.Semantic proximity Read ↗

Detection of Anti-Hepatitis B Virus Drug Resistance Mutations Based on Multicolor Melting Curve Analysis.Semantic proximity Read ↗

Multiplex locked nucleic acid probes for analysis of hepatitis B virus mutants using real-time PCR.Semantic proximity Read ↗

Ultrasensitive quantification of hepatitis B virus A1762T/G1764A mutant by a SimpleProbe PCR using a wild-type-selective PCR blocker and a primer-blocker-probe partial-overlap approach.Semantic proximity Read ↗

Simultaneous quantitation and genotyping of hepatitis B virus by real-time PCR and melting curve analysis.Semantic proximity Read ↗

Mutation detection using fluorescent hybridization probes and melting curve analysis.Semantic proximity Read ↗

Simultaneous quantification and genotyping of hepatitis B virus for genotypes A to G by real-time PCR and two-step melting curve analysis.Semantic proximity Read ↗

New real-time-PCR method to identify single point mutations in hepatitis C virus.Semantic proximity Read ↗

Improved TaqMan real-time assays for detecting hepatitis A virus.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.