Article
A real-time Taqman method for hepatitis C virus genotyping.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Oct 2005
Rolfe Kathryn J, Alexander Graeme J M, Wreghitt Tim G, Parmar Surendra, Jalal Hamid, Curran Martin D
Abstract excerpt
BACKGROUND: There is the need for a rapid, inexpensive method for genotyping hepatitis C virus (HCV) to support clinical practice. OBJECTIVES: To develop a real-time (Rotor-Gene 3000) Taqman assay for HCV genotyping in a single tube. STUDY DESIGN: Seven type-specific probes, two for genotypes 1-3 and one for genotype 4 were designed around genotype-specific motifs in the 5' non-coding (NC) region to create two...
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