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Article

Detection of Anti-Hepatitis B Virus Drug Resistance Mutations Based on Multicolor Melting Curve Analysis.

Journal of clinical microbiology - 1 Nov 2016

Mou Yi, Athar Muhammad Ammar, Wu Yuzhen, Xu Ye, Wu Jianhua, Xu Zhenxing, Hayder Zulfiqar, Khan Saeed, Idrees Muhammad, Nasir Muhammad Israr, Liao Yiqun, Li Qingge

Abstract excerpt

Detection of anti-hepatitis B virus (HBV) drug resistance mutations is critical for therapeutic decisions for chronic hepatitis B virus infection. We describe a real-time PCR-based assay using multicolor melting curve analysis (MMCA) that could accurately detect 24 HBV nucleotide mutations at 10 amino acid positions in the reverse transcriptase region of the HBV polymerase gene. The two-reaction assay had a limit...

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Identifiers

PMID: 27535686
DOI: 10.1128/JCM.00439-16
PMCID: PMC5078540

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