Article
Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis.
Protein engineering - 1 May 1999
Miyazaki C, Iba Y, Yamada Y, Takahashi H, Sawada J, Kurosawa Y
Abstract excerpt
The specificity for 11-deoxycortisol (11-DOC) of a monoclonal antibody (mAb), designated SCET, was changed to specificity for cortisol (CS) by site-specific mutagenesis followed by random mutagenesis. The Fab form of SCET was expressed on the surface of a phage. During the first step, mutations were introduced at 14 amino acid positions in three complementarity-determining regions (CDRs) of the VH domain that...
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