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Article

Fine tuning of the specificity of an anti-progesterone antibody by first and second sphere residue engineering.

The Journal of biological chemistry - 1 Jul 2005

Dubreuil Olivier, Bossus Marc, Graille Marc, Bilous Maëlle, Savatier Alexandra, Jolivet Michel, Ménez André, Stura Enrico, Ducancel Frédéric

Abstract excerpt

The specificity of anti-progesterone P15G12C12G11 antibody was improved by combination of in vitro scanning saturation mutagenesis and error-prone PCR. The most evolved mutant is able to discriminate against 5beta- or 5alpha-dihydroprogesterone, 23 and 15 times better than the starting antibody, while maintaining the affinity for progesterone that remains in the picomolar range. The high level of homology with...

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Identifiers

PMID: 15878862
DOI: 10.1074/jbc.M500048200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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