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Article

Altered hapten recognition by two anti-digoxin hybridoma variants due to variable region point mutations.

The Journal of biological chemistry - 5 Mar 1991

Schildbach J F, Panka D J, Parks D R, Jager G C, Novotny J, Herzenberg L A, Mudgett-Hunter M, Bruccoleri R E, Haber E, Margolies M N

Abstract excerpt

Two spontaneous variants of the murine anti-digoxin antibody-producing hybridoma cell line 26-10 were isolated by two-color fluorescence-activated cell sorting on the basis of altered hapten binding. The variable region sequences of the antibodies produced by the mutant lines revealed that each contains a single amino acid change in the heavy chain second complementarity determining region. A Tyr to His change at...

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PMID: 1999439

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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