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Article

Altered hapten recognition by two anti-digoxin hybridoma variants due to variable region point mutations.

The Journal of biological chemistry - 5 Mar 1991

Schildbach J F, Panka D J, Parks D R, Jager G C, Novotny J, Herzenberg L A, Mudgett-Hunter M, Bruccoleri R E, Haber E, Margolies M N

Abstract excerpt

Two spontaneous variants of the murine anti-digoxin antibody-producing hybridoma cell line 26-10 were isolated by two-color fluorescence-activated cell sorting on the basis of altered hapten binding. The variable region sequences of the antibodies produced by the mutant lines revealed that each contains a single amino acid change in the heavy chain second complementarity determining region. A Tyr to His change at...

Topics

Amino Acid Sequence
Antibodies, Monoclonal
Antibody Specificity
Base Sequence
Digoxigenin
Digoxin
Genes, Immunoglobulin
Haptens
Hybridomas
Immunoglobulin Idiotypes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Models, Molecular

Molecular Sequence Data

Mutation

Ouabain

Structure-Activity Relationship

Source

Identifiers

PMID: 1999439

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1999439-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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