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The VH framework region 1 as a target of efficient mutagenesis for generating a variety of affinity-matured scFv mutants

15 Apr 2021

Abstract excerpt

Abstract In vitro affinity-maturation potentially generates antibody fragments with enhanced antigen-binding affinities that allow for developing more sensitive diagnostic systems and more effective therapeutic agents. Site-directed mutagenesis targeting “hot regions,” i.e., amino acid substitutions therein frequently increase the affinities, is desirable for straightforward discovery of valuable mutants. We here...

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PMID: 33859250
DOI: 10.1038/s41598-021-87501-7

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