Article
A novel simple and rapid PCR-based site-directed mutagenesis method.
Molecular biotechnology - 1 Jan 2004
Rabhi Imen, Guedel Naouel, Chouk Imen, Zerria Khaled, Barbouche M Ridha, Dellagi Koussay, Fathallah Dahmani M
Abstract excerpt
Site-directed mutagenesis (SDM) is a powerful tool for exploring protein structure and function, and several procedures adjusted to specific purposes are still being developed. Herein we describe a straightforward and efficient method with versatile applications for introducing site-specific alterations in any deoxyribonucleic acid (DNA) sequence cloned in a plasmidic expression vector. In this polymerase chain...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.