Article
Fine tuning of an anti-testosterone antibody binding site by stepwise optimisation of the CDRs.
Immunotechnology : an international journal of immunological engineering - 1 Jun 1998
Hemminki A, Niemi S, Hautoniemi L, Söderlund H, Takkinen K
Abstract excerpt
BACKGROUND: We have previously reported specificity improvement of an anti-testosterone monoclonal antibody (3-C4F5) by random mutagenesis of the third complementarity determining regions (CDR3s) and by phage display selection. OBJECTIVES: Here we extend the mutagenesis strategy to the other CDRs...
Topics
- Antibody Affinity
- Binding Sites, Antibody
- Cloning, Molecular
- Cross Reactions
- Dehydroepiandrosterone
- Evaluation Studies as Topic
- Gene Library
- Humans
- Immunoassay
- Immunoglobulin Fab Fragments
- Immunoglobulin Variable Region
- Kinetics
- Mutagenesis
- Mutation
- Testosterone