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Article

Switching antibody specificity through minimal mutation.

Journal of molecular biology - 4 Apr 2008

Piatesi Andrea, Aldag Caroline, Hilvert Donald

Abstract excerpt

Antibody 1E9, which was elicited with a hexachloronorbornene derivative and catalyzes the Diels-Alder reaction between tetrachlorothiophene dioxide and N-ethylmaleimide with high efficiency, was successfully reengineered to bind a range of structurally diverse steroids with nanomolar affinities. Remarkably, two mutations (Leu(H47)Trp/Arg(H100)Trp) out of 36 total sequence differences suffice to switch the...

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Share to Cross Reactions
Share to Antibodies, Monoclonal
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Mutagenesis, Site-Directed

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Source

Identifiers

PMID: 18295792
DOI: 10.1016/j.jmb.2008.01.069

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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