Article
Evidence for dimerization of dimers in K+ channel assembly.
Biophysical journal - 1 Apr 1999
Tu L, Deutsch C
Abstract excerpt
Voltage-gated K+ channels are tetrameric, but how the four subunits assemble is not known. We analyzed inactivation kinetics and peak current levels elicited for a variety of wild-type and mutant Kv1.3 subunits, expressed singly, in combination, and as tandem constructs, to show that 1) the dominant pathway involves a dimerization of dimers, and 2) dimer-dimer interaction may involve interaction sites that differ...
Topics
- Animals
- Binding Sites
- Biophysical Phenomena
- Biophysics
- Dimerization
- Female
- In Vitro Techniques
- Kinetics
- Membrane Potentials
- Models, Biological
- Mutation
- Neuropeptides
- Oocytes
- Potassium Channels
- Potassium Channels, Voltage-Gated
- Protein Conformation
- Recombinant Proteins
- Shaw Potassium Channels