Article

A synthetic RNA-mediated evolution system in yeast

2021-02-28

Abstract excerpt

Laboratory evolution is a powerful approach to search for genetic adaptations to new or improved phenotypes, yet either relies on labour-intensive human-guided iterative rounds of mutagenesis and selection, or prolonged adaptation regimes based on naturally evolving cell populations. Here we present CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) of genomic loci using evolving chimeric donor gRNAs co...

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Literature Corpus work: fc41e879-f509-59ea-894d-d875406928e5
DOI: 10.1101/2021.02.27.433199

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