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Article

A synthetic RNA-mediated evolution system in yeast.

Nucleic acids research - 7 Sept 2021

Jensen Emil D, Laloux Marcos, Lehka Beata J, Pedersen Lasse E, Jakočiūnas Tadas, Jensen Michael K, Keasling Jay D

Abstract excerpt

Laboratory evolution is a powerful approach to search for genetic adaptations to new or improved phenotypes, yet either relies on labour-intensive human-guided iterative rounds of mutagenesis and selection, or prolonged adaptation regimes based on naturally evolving cell populations. Here we present CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) of genomic loci using evolving chimeric donor gRNAs...

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Identifiers

PMID: 34107026
DOI: 10.1093/nar/gkab472
PMCID: PMC8421215

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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