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Article

Single-cell RNA counting at allele and isoform resolution using Smart-seq3.

Nature biotechnology - 1 Jun 2020

Hagemann-Jensen Michael, Ziegenhain Christoph, Chen Ping, Ramsköld Daniel, Hendriks Gert-Jan, Larsson Anton J M, Faridani Omid R, Sandberg Rickard

Abstract excerpt

Large-scale sequencing of RNA from individual cells can reveal patterns of gene, isoform and allelic expression across cell types and states1. However, current short-read single-cell RNA-sequencing methods have limited ability to count RNAs at allele and isoform resolution, and long-read sequencing techniques lack the depth required for large-scale applications across cells2,3. Here we introduce Smart-seq3, which...

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Identifiers

PMID: 32518404
DOI: 10.1038/s41587-020-0497-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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