Article

Integrating short-read and long-read single-cell RNA sequencing for comprehensive transcriptome profiling in mouse retina

2024-02-23

Abstract excerpt

The vast majority of protein-coding genes in the human genome produce multiple mRNA isoforms through alternative splicing, significantly enhancing the complexity of the transcriptome and proteome. To establish an efficient method for characterizing transcript isoforms within tissue samples, we conducted a systematic comparison between single-cell long-read and conventional short-read RNA sequencing techniques. The...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 192ba5ed-fe69-58d3-88e2-d4d5ededeed2
DOI: 10.1101/2024.02.20.581234

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Integrating short-read and long-read single-cell RNA sequencing for comprehensive transcriptome profiling in mouse retinaDOI 10.1101/2024.02.20.581234Read

Long-read single cell transcriptomics uncovers isoform preferences in developing human retinaSemantic proximity Read

Iso-Seq enables discovery of novel isoform variants in human retina at single cell resolutionSemantic proximity Read

A technology-agnostic long-read analysis pipeline for transcriptome discovery and quantificationSemantic proximity Read

Dynamic changes in mRNA isoform usage during human retinal organoid developmentSemantic proximity Read

Comprehensive characterization of single cell full-length isoforms in human and mouse with long-read sequencingSemantic proximity Read

Full-length transcript sequencing of human and mouse identifies widespread isoform diversity and alternative splicing in the cerebral cortexSemantic proximity Read

Single-cell RNA counting at allele- and isoform-resolution using Smart-seq3Semantic proximity Read

The ENCODE4 long-read RNA-seq collection reveals distinct classes of transcript structure diversitySemantic proximity Read

Single-cell RNA counting at allele and isoform resolution using Smart-seq3.Semantic proximity Read

Cell-specific RNA isoform remodeling in the aging mouse brainSemantic proximity Read

Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencingSemantic proximity Read

scCAT-seq:single-cell identification and quantification of mRNA isoforms by cost-effective short-read sequencing of cap and tailSemantic proximity Read