Article

Optimisation of immunocytochemistry methodology for the detection of endogenous eIF2B localised foci

2022-12-08

Abstract excerpt

The multisubunit eukaryotic initiation factor 2B (eIF2B), a guanine nucleotide exchange factor (GEF) for eIF2, is an essential regulator of translation initiation. Activation of the cellular integrated stress response (ISR) by factors such as endoplasmic reticulum stress leads to phosphorylation of eIF2α and inhibition of eIF2B GEF activity. Cytoplasmic bodies containing eIF2B subunits, termed eIF2B bodies, have b...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: fa3c1128-561c-592c-9efb-2ce66986584e
DOI: 10.1101/2022.12.06.519266

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Optimisation of immunocytochemistry methodology for the detection of endogenous eIF2B localised fociDOI 10.1101/2022.12.06.519266Read

eIF2Bα subcellular localisation – a potential link between translation initiation and stress granule formation?Semantic proximity Read

eIF2B localisation and its regulation during the integrated stress response is cell type specificSemantic proximity Read

Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.Semantic proximity Read

A molecule-inhibitor of the integrated stress response regulates activity of mammalian eukaryotic translation initiation factor 2BSemantic proximity Read

Mutational analysis of the alpha subunit of eIF2B provides insights into the role of eIF2B bodies in translational control and VWM disease.Semantic proximity Read

Identification of new interactors of eIF3f by endogenous proximity-dependent biotin labelling in human muscle cellsSemantic proximity Read

Insights into the architecture of the eIF2Bα/β/δ regulatory subcomplex.Semantic proximity Read

Structure of the nucleotide exchange factor eIF2B reveals mechanism of memory-enhancing molecule.Semantic proximity Read

Structural Roles for Human Translation Factor eIF3 in Initiation of Protein SynthesisSemantic proximity Read

Homologous segments in three subunits of the guanine nucleotide exchange factor eIF2B mediate translational regulation by phosphorylation of eIF2.Semantic proximity Read

The N-terminal domain of the human eIF2beta subunit and the CK2 phosphorylation sites are required for its function.Semantic proximity Read

Expression of fragments of translation initiation factor eIF4GI reveals a nuclear localisation signal within the N-terminal apoptotic cleavage fragment N-FAG.Semantic proximity Read