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Article

Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.

Clinical chemistry - 1 Jan 2015

Castellanos-Rizaldos Elena, Richardson Katherine, Lin Rui, Wu Grant, Makrigiorgos Mike G

Abstract excerpt

BACKGROUND: Multiplexed detection of low-level mutations presents a technical challenge for many technologies, including cancer gene panels used for targeted-resequencing. Analysis of mutations below approximately 2%-5% abundance in tumors with heterogeneity, samples with stromal contamination, or biofluids is problematic owing to increased noise from sequencing errors. Technologies that reduce noise via deep...

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Identifiers

PMID: 25297854
DOI: 10.1373/clinchem.2014.228361
PMCID: PMC4281501

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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