Article
An AsCas12f-based compact genome-editing tool derived by deep mutational scanning and structural analysis.
Cell - 26 Oct 2023
Hino Tomohiro, Omura Satoshi N, Nakagawa Ryoya, Togashi Tomoki, Takeda Satoru N, Hiramoto Takafumi, Tasaka Satoshi, Hirano Hisato, Tokuyama Takeshi, Uosaki Hideki, Ishiguro Soh, Kagieva Madina, Yamano Hiroyuki, Ozaki Yuki, Motooka Daisuke, Mori Hideto, Kirita Yuhei, Kise Yoshiaki, Itoh Yuzuru, Matoba Satoaki, Aburatani Hiroyuki, Yachie Nozomu, Karvelis Tautvydas, Siksnys Virginijus, Ohmori Tsukasa, Hoshino Atsushi, Nureki Osamu
Abstract excerpt
SpCas9 and AsCas12a are widely utilized as genome-editing tools in human cells. However, their relatively large size poses a limitation for delivery by cargo-size-limited adeno-associated virus (AAV) vectors. The type V-F Cas12f from Acidibacillus sulfuroxidans is exceptionally compact (422 amino acids) and has been harnessed as a compact genome-editing tool. Here, we developed an approach, combining deep...
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