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Why Does the E1219V Mutation Expand T-Rich PAM Recognition in Cas9 from Streptococcus pyogenes?

Journal of chemical information and modeling - 22 Apr 2024

Bhattacharya Shreya, Satpati Priyadarshi

Abstract excerpt

Popular RNA-guided DNA endonuclease Cas9 from Streptococcus pyogenes (SpCas9) recognizes the canonical 5'-NGG-3' protospacer adjacent motif (PAM) and triggers double-stranded DNA cleavage activity. Mutations in SpCas9 were demonstrated to expand the PAM readability and hold promise for therapeutic and genome editing applications. However, the energetics of the PAM recognition and its relation to the atomic...

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PMID: 38600752
DOI: 10.1021/acs.jcim.3c01515

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