Article

Activation mechanism and molecular engineering of Staphylococcus aureus Cas9

2025-09-22

Abstract excerpt

<title>Abstract</title> <p> <italic>Staphylococcus aureus</italic> Cas9 (SaCas9) is smaller than the widely used <italic>Streptococcus pyogenes</italic> Cas9 (SpCas9) and has been harnessed for gene therapy using an adeno-associated virus vector. However, SaCas9 requires a longer NNGRRT (where N is any nucleotide and R is A or G) protospacer adjacent motif (PAM) for target DNA recognition, thereby restricting...

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Literature Corpus work: dd353dd7-d4cc-5d36-a056-b385784efb95
DOI: 10.21203/rs.3.rs-7614961/v1

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