Article
Enabling large-scale genome editing at repetitive elements by reducing DNA nicking.
Nucleic acids research - 21 May 2020
Smith Cory J, Castanon Oscar, Said Khaled, Volf Verena, Khoshakhlagh Parastoo, Hornick Amanda, Ferreira Raphael, Wu Chun-Ting, Güell Marc, Garg Shilpa, Ng Alex H M, Myllykallio Hannu, Church George M
Abstract excerpt
To extend the frontier of genome editing and enable editing of repetitive elements of mammalian genomes, we made use of a set of dead-Cas9 base editor (dBE) variants that allow editing at tens of thousands of loci per cell by overcoming the cell death associated with DNA double-strand breaks and single-strand breaks. We used a set of gRNAs targeting repetitive elements-ranging in target copy number from about 32...
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