Article

MUTE-Seq: An Ultrasensitive Method for Detecting Low-Frequency Mutations in ctDNA with Engineered Advanced-Fidelity FnCas9

2024-02-21

Abstract excerpt

<title>Abstract</title> <p>In this study, we present the development of the Mutation tagging by CRISPR-based Ultra-precise Targeted Elimination in Sequencing (MUTE-Seq) method. We engineered a highly precise advanced-fidelity FnCas9 variant, named FnCas9-AF2, to effectively discriminate single-base mismatches at all positions of the single guide RNA (sgRNA) target sequences. FnCas9-AF2 exhibited significantly low...

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Literature Corpus work: f0f04939-f6c1-5182-a281-e4dd95786278
DOI: 10.21203/rs.3.rs-3313031/v1

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