Article
New applications of CRISPR/Cas9 system on mutant DNA detection.
Gene - 30 Jan 2018
Jia Chenqiang, Huai Cong, Ding Jiaqi, Hu Lingna, Su Bo, Chen Hongyan, Lu Daru
Abstract excerpt
The detection of mutant DNA is critical for precision medicine, but low-frequency DNA mutation is very hard to be determined. CRISPR/Cas9 is a robust tool for in vivo gene editing, and shows the potential for precise in vitro DNA cleavage. Here we developed a DNA mutation detection system based o...
Topics
- Amplified Fragment Length Polymorphism Analysis
- CRISPR-Cas Systems
- Carcinoma, Non-Small-Cell Lung
- DNA
- Deoxyribonuclease I
- ErbB Receptors
- HEK293 Cells
- Hemoglobin Subunits
- Humans
- Lung Neoplasms
- Mutation
- Mutation Rate
- Polymerase Chain Reaction
- Tumor Cells, Cultured