Article
MUTE-Seq: An Ultrasensitive Method for Detecting Low-Frequency Mutations in cfDNA With Engineered Advanced-Fidelity FnCas9.
Advanced materials (Deerfield Beach, Fla.) - 1 Nov 2025
Ye Sunghyeok, Kim Jin-Soo, Kim Myungshin, Kim Ki-Yeon, Won Yoon-Ho, Park Taegun, An Sungjae, Jeong Haerin, Chung Hee-Joon, Lee In Seon, Kang Myoung-Hee, Kang Chan Young, Kim Mi Young, Chung Jae Ho, Gim Jeong-An, Hwang Woochang, Kim Yonggoo, Kim Song Cheol, Lee Sungho, Hur Junho K, Hur Junseok W
Abstract excerpt
In this study, we present the development of the Mutation tagging by CRISPR-based Ultra-precise Targeted Elimination in Sequencing (MUTE-Seq) method. We engineered a highly precise advanced-fidelity FnCas9 variant, named FnCas9-AF2, to effectively discriminate single-base mismatches at all positions of the single guide RNA (sgRNA) target sequences. FnCas9-AF2 exhibited significantly lower off-target effects...
Topics
- Humans
- Mutation
- CRISPR-Cas Systems
- Carcinoma, Non-Small-Cell Lung
- Cell-Free Nucleic Acids
- Lung Neoplasms
- Leukemia, Myeloid, Acute
- Pancreatic Neoplasms
- RNA, Guide, CRISPR-Cas Systems