Article

Protein-mediated stabilization and nicking of the non-template DNA strand dramatically affect R-loop formation <i>in vitro</i>

2025-04-26

Abstract excerpt

<h4>ABSTRACT</h4> R-loops are an important class of non-B DNA structures that form co-transcriptionally. Using in vitro transcription and unbiased quantitative sequencing readouts, we show that the addition of single-strand DNA binding proteins co-transcriptionally can drive a 3- to 5-fold increase of R-loop frequency without significant changes to R-loop distribution. We propose that this is caused by stabilizi...

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Identifiers and source

Literature Corpus work: eb098d68-e0c9-58e2-9534-edadfe008c5f
DOI: 10.1101/2025.04.24.649451

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Protein-mediated stabilization and nicking of the non-template DNA strand dramatically affect R-loop formation <i>in vitro</i>DOI 10.1101/2025.04.24.649451Read

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