Article

A peptide-centric local stability assay to unveil protein targets of diverse ligands

2023-10-19

Abstract excerpt

<h4>ABSTRACT</h4> While tremendous progress has been made in chemical proteomics for identifying protein-ligand interactions, it remains challenging for proteome-wide identification of ligand-binding regions without modifying the ligands. Here, we discovered that “disruptive trypsinization” amplifies the readout of ligand-induced protein local stability shifts, and explored this notion in developing “peptide-cent...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: ea43b126-6695-5e33-a54b-b8176f5fee01
DOI: 10.1101/2023.10.17.562693

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A peptide-centric local stability assay to unveil protein targets of diverse ligandsDOI 10.1101/2023.10.17.562693Read

Human SRMAtlas: A Resource of Targeted Assays to Quantify the Complete Human Proteome.Semantic proximity Read

A Two-Dimensional Peptide-Centric Stability Assay Maps Multidimensional Target Landscapes Unveiling 17β-Estradiol (E2)-GPX4 Binding-Induced FerroptosisSemantic proximity Read

Reimagining proteome-wide evaluation of residue-specific bioconjugation chemistriesSemantic proximity Read

System-wide identification and prioritization of enzyme substrates by thermal analysis (SIESTA)Semantic proximity Read

Ninhydrin as a covalent warhead for chemical proteomic-enabled discovery and selective engagement of reactive argininesSemantic proximity Read

Next-Generation Multiplexed Targeted Proteomics Quantifies Post-Translational Modifications, Compound-Protein Interactions, and Disease Biomarkers with High ThroughputSemantic proximity Read

A Global Ligandability Map of Tryptoline Butynamide Stereoprobes Identifies Covalent Inhibitors of the Actin Maturation Protease ACTMAPSemantic proximity Read

Deep thermal proteome profiling for detection of proteoforms and drug sensitivity biomarkersSemantic proximity Read

Target deconvolution of HDAC pharmacopoeia highlights MBLAC2 as common off-targetSemantic proximity Read

Assigning functionality to cysteines by base editing of cancer dependency genesSemantic proximity Read

Machine learning-based exploration of enzyme-substrate networks: SET8-mediated methyllysine and its changing impact within cancer proteomesSemantic proximity Read

DPHL v2: An updated and comprehensive DIA pan-human assay library for quantifying more than 14,000 proteinsSemantic proximity Read