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A Two-Dimensional Peptide-Centric Stability Assay Maps Multidimensional Target Landscapes Unveiling 17β-Estradiol (E2)-GPX4 Binding-Induced Ferroptosis

2025-07-04

Abstract excerpt

<title>Abstract</title> <p>Current methods for mapping ligand-protein interactions struggle to resolve weak but critical binding events and conformational dynamics, particularly for endogenous hormones with pleiotropic functions like 17β-estradiol (E2). In this study, we developed a two-dimensional (2D) PEptide-centric Local Stability Assay (PELSA). By detecting increasing ligand dose-induced local binding rates...

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Literature Corpus work: 8250dc7b-f8da-5d50-9ee2-23d948d3f9d2
DOI: 10.21203/rs.3.rs-6893001/v1

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A Two-Dimensional Peptide-Centric Stability Assay Maps Multidimensional Target Landscapes Unveiling 17β-Estradiol (E2)-GPX4 Binding-Induced FerroptosisDOI 10.21203/rs.3.rs-6893001/v1Read

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