Article

Calibration-free NGS Quantitation of Mutations below 0.01% VAF

2021-06-10

Abstract excerpt

<title>Abstract</title> <p>The quantitation of rare somatic mutations is essential for basic research and translational clinical applications including minimal residual disease (MRD) detection. Though unique molecular identifier (UMI) has suppressed sequencing error and allowed detection rare mutation, the sequencing depth requirement is high. The blocker displacement amplification (BDA) allele enrichment method...

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Literature Corpus work: ea40970e-b02b-5a0c-8bee-bac5fda5f5fc
DOI: 10.21203/rs.3.rs-579121/v1

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