Article

Structural basis for gene silencing by siRNAs in humans

2024-12-06

Abstract excerpt

<h4>Summary</h4> Small interfering RNAs (siRNAs) guide mRNA cleavage by human Argonaute2 (hAgo2), leading to targeted gene silencing. Despite their laboratory and clinical impact, structural insights into human siRNA catalytic activity remain elusive. Here, we show that disrupting siRNA 3’-end binding by hAgo2 accelerates target cleavage and stabilizes its catalytic conformation, enabling detailed structural anal...

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Literature Corpus work: e42d0608-5a9d-5a99-8714-c2a5f258fe2c
DOI: 10.1101/2024.12.05.627081

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