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Article

Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing.

Cell stem cell - 3 Jul 2014

Veres Adrian, Gosis Bridget S, Ding Qiurong, Collins Ryan, Ragavendran Ashok, Brand Harrison, Erdin Serkan, Cowan Chad A, Talkowski Michael E, Musunuru Kiran

Abstract excerpt

Genome editing has attracted wide interest for the generation of cellular models of disease using human pluripotent stem cells and other cell types. CRISPR-Cas systems and TALENs can target desired genomic sites with high efficiency in human cells, but recent publications have led to concern about the extent to which these tools may cause off-target mutagenic effects that could potentially confound...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24996167
DOI: 10.1016/j.stem.2014.04.020
PMCID: PMC4082799

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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