Article

CRISPR screen for rAAV production implicates genes associated with infection

2024-09-18

Abstract excerpt

<h4>ABSTRACT</h4> Recombinant adeno-associated virus (rAAV) vectors are an effective and well-established tool in the growing gene therapy field, with five FDA-approved AAV-mediated gene therapies already on the market and numerous more in clinical trials. However, manufacturing rAAV vectors is an expensive, timely, and labor-intensive process that limits the commercial use of AAV-mediated gene therapies. To addr...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: e37d3149-4cfe-57d2-bac9-cd9900507694
DOI: 10.1101/2024.09.17.613356

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

CRISPR screen for rAAV production implicates genes associated with infectionDOI 10.1101/2024.09.17.613356Read

Genome-wide activation screens to increase adeno-associated virus productionSemantic proximity Read

Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral VectorsSemantic proximity Read

Advancing AAV production with high-throughput screening and transcriptomicsSemantic proximity Read

AAV-Based Gene Therapy: Opportunities, Risks, and Scale-Up StrategiesSemantic proximity Read

Unravelling the essential elements for recombinant adeno-associated virus (rAAV) production in animal cell-based platformsSemantic proximity Read

Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12aSemantic proximity Read

Genome-scale analysis of cellular restriction factors that inhibit transgene expression from adeno-associated virus vectorsSemantic proximity Read

Future of rAAV Gene Therapy: Platform for RNAi, Gene Editing, and BeyondSemantic proximity Read

Pooled Screens Identify GPR108 and TM9SF2 as Host Cell Factors Critical for AAV TransductionSemantic proximity Read

Trojan Horse-Like Vehicles for CRISPR-Cas Delivery: Engineering Extracellular Vesicles and Virus-Like Particles for Precision Gene Editing in Cystic FibrosisSemantic proximity Read

Production of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293 cellsSemantic proximity Read

rAAV capsid mutants eliminate leaky expression from DNA donor template for homologous recombination.Semantic proximity Read