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Article

rAAV capsid mutants eliminate leaky expression from DNA donor template for homologous recombination.

Nucleic acids research - 24 Jun 2024

Ling Chen, Yu Chenghui, Wang Cong, Yang Ming, Yang Hengbin, Yang Keying, He Yun, Shen Yajie, Tang Shiyi, Yu Xiaomin, Zhou Zhengjun, Zhou Shaolai, Zhou Jian, Zhu Liqing, Li Jixi

Abstract excerpt

Precise genomic editing through the combination of CRISPR/Cas systems and recombinant adeno-associated virus (rAAV)-delivered homology directed repair (HDR) donor templates represents a powerful approach. However, the challenge of effectively suppressing leaky transcription from the rAAV vector, a phenomenon associated to cytotoxicity, persists. In this study, we demonstrated substantial promoter activities of...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 38783157
DOI: 10.1093/nar/gkae401
PMCID: PMC11194064

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VCP

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