Article

Rapid Adeno-Associated Virus Genome Quantification with Amplification-Free CRISPR-Cas12a

2023-11-15

Abstract excerpt

Efficient manufacturing of recombinant Adeno-Associated Viral (rAAV) vectors to meet rising clinical demand remains a major hurdle. One of the most significant challenges is the generation of large amounts of empty capsids without the therapeutic genome. There is no standardized analytical method to accurately quantify the viral genes, and subsequently the empty-to-full ratio, making the manufacturing challenges e...

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Literature Corpus work: fba2ef43-cc95-5bf0-a54f-f2bad5f30886
DOI: 10.1101/2023.11.14.567134

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