CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method

Abstract excerpt

This study develops a new method for detecting and typing target DNA based on Cas9 nuclease, which was named as ctPCR, representing Cas9/sgRNA- or CRISPR-typing PCR. The technique can detect and discriminate target DNA easily, rapidly, specifically, and sensitively. This technique detects target DNA in three steps: (1) amplifying target DNA with PCR by using a pair of universal primers (PCR1); (2) treating PCR1 pr...

Topics

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• Gene Editing
• Human papillomavirus 16
• Human papillomavirus 18
• Oligonucleotides
• RNA, Guide, CRISPR-Cas Systems

Identifiers and source

Literature Corpus work

ab8b0be5-c320-5881-9290-a25a57fe5399

DOI

10.1101/236588