Article

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

2017-07-06

Abstract excerpt

The RNA-guided CRISPR-Cas9 nuclease from Streptococcus pyogenes (SpCas9) has been widely repurposed for genome editing 1-4 . High-fidelity (SpCas9-HF1) and enhanced specificity (eSpCas9(1.1)) variants exhibit substantially reduced off-target cleavage in human cells, but the mechanism of target discrimination and the potential to further improve fidelity were unknown 5-9 . Using single-molecule Förster resonance...

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Literature Corpus work: e13213df-7c92-542a-8c21-ec3389dd549d
DOI: 10.1101/160036

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