Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.

Nature - 19 Oct 2017

Chen Janice S, Dagdas Yavuz S, Kleinstiver Benjamin P, Welch Moira M, Sousa Alexander A, Harrington Lucas B, Sternberg Samuel H, Joung J Keith, Yildiz Ahmet, Doudna Jennifer A

Abstract excerpt

The RNA-guided CRISPR-Cas9 nuclease from Streptococcus pyogenes (SpCas9) has been widely repurposed for genome editing. High-fidelity (SpCas9-HF1) and enhanced specificity (eSpCas9(1.1)) variants exhibit substantially reduced off-target cleavage in human cells, but the mechanism of target discrimination and the potential to further improve fidelity are unknown. Here, using single-molecule Förster resonance energy...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biotechnology
Share to CRISPR-Associated Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CRISPR-Cas Systems

Share to Endonucleases

Share to Enzyme Activation

Share to Gene Editing

Share to Protein Domains

Share to Streptococcus pyogenes

Source

Open in PubMed Open full text

Identifiers

PMID: 28931002
DOI: 10.1038/nature24268
PMCID: PMC5918688

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.PMID 28931002Read ↗

Enhanced proofreading governs CRISPR-Cas9 targeting accuracySemantic proximity Read ↗

High-fidelity SaCas9 identified by directional screening in human cells.Semantic proximity Read ↗

Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.Semantic proximity Read ↗

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.Semantic proximity Read ↗

Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis.Semantic proximity Read ↗

Quantifying Protein-Nucleic Acid Interactions for Engineering Useful CRISPR-Cas9 Genome-Editing Variants.Semantic proximity Read ↗

Coordinated Actions of Cas9 HNH and RuvC Nuclease Domains Are Regulated by the Bridge Helix and the Target DNA Sequence.Semantic proximity Read ↗

A highly specific SpCas9 variant is identified by in vivo screening in yeast.Semantic proximity Read ↗

Differential Allosteric Modulation of Cas9 Specificity.Semantic proximity Read ↗

Decoupling the bridge helix of Cas12a results in a reduced trimming activity, increased mismatch sensitivity and impaired conformational transitions.Semantic proximity Read ↗

Combination of engineered FnCas9 and extended gRNAs for PAM-flexible, robust and nucleobase specific editing and diagnosticsSemantic proximity Read ↗

Designing Fidelity of CRISPR-Cas Endonucleases by Kinetic InsightsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.