Article

Designing Fidelity of CRISPR-Cas Endonucleases by Kinetic Insights

2026-07-07

Abstract excerpt

<h4>Finding: </h4> high-fidelity CRISPR-Cas variants is critical for both the precision of in vitro DNA detection and the safety of in vivo gene-editing therapeutics. However, the large size of the Cas enzyme and the distinct selection criteria between its natural evolution and clinical practice lead to extensive experimental trials and limited success rate for de novo design, directed evolution, protein language...

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Literature Corpus work: 7502a770-4a39-5676-ae05-b90d9711f55e
DOI: 10.64898/2026.07.06.736881

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