Article

Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens

2020-08-21

Abstract excerpt

CRISPR-Cas13 mediates robust transcript knockdown in human cells through direct RNA targeting. Compared to DNA-targeting CRISPR enzymes like Cas9, RNA targeting by Cas13 is transcript- and strand-specific: It can distinguish and specifically knock-down processed transcripts, alternatively spliced isoforms and overlapping genes, all of which frequently serve different functions. Previously, we identified optimal de...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 3838b444-693d-53f9-b835-fb126f7e5a82
DOI: 10.1101/2020.08.20.259762

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogensDOI 10.1101/2020.08.20.259762Read

Principles for rational Cas13d guide designSemantic proximity Read

CRISPR/Cas13-Based Anti-RNA Viral ApproachesSemantic proximity Read

CRISPR/Cas13-Based Anti-RNA Viral ApproachesSemantic proximity Read

Deep learning and CRISPR-Cas13d ortholog discovery for optimized RNA targetingSemantic proximity Read

Programmable Inhibition and Detection of RNA Viruses Using Cas13Semantic proximity Read

Engineering CRISPR/Cas13 System against RNA Viruses: From Diagnostics to TherapeuticsSemantic proximity Read

A comprehensive analysis and resource to use CRISPR-Cas13 for broad-spectrum targeting of RNA virusesSemantic proximity Read

RNA editing with CRISPR-Cas13Semantic proximity Read

Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxSemantic proximity Read

Customizable host and viral transcript enrichment using CRISPR-Cas9 long-read sequencing for isoform discovery and validationSemantic proximity Read

Machine learning methods for predicting guide RNA effects in CRISPR epigenome editing experimentsSemantic proximity Read

Optimizing a CRISPR-Cas13d gene circuit for tunable target RNA downregulation with minimal collateral RNA cuttingSemantic proximity Read