Article

A synthetic CRISPR-Cas nuclease with expanded enzymatic activities

2023-11-12

Abstract excerpt

<h4>Summary</h4> Clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonucleases have revolutionized biotechnology for their potential as programmable genome editors. Yet, most natural nucleases and their variants have limitations. Here, we report a fully synthetic CRISPR-associated (Cas) nuclease (α-synCas) designed by Ancestral Sequence Reconstruction (ASR) that displays a set of rob...

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Literature Corpus work: 0c9cd029-8b60-5d19-906c-5afaa7525e3d
DOI: 10.1101/2023.11.12.566739

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