Article

Editing of the TRIM5 gene decreases the permissiveness of human T lymphocytic cells to HIV-1

2020-12-08

Abstract excerpt

TRIM5α is a cytoplasmic antiretroviral effector upregulated by type I interferons (IFN-I). We previously showed that two points mutations, R332G/R335G, in the retroviral capsid-binding region confer human TRIM5α the capacity to target and strongly restrict HIV-1 upon over-expression of the mutated protein. Here, we used CRISPR-Cas9-mediated homology-directed repair (HDR) to introduce these two mutations in the end...

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Literature Corpus work: d7a90998-3397-5694-b2b6-c6b090925e4d
DOI: 10.1101/2020.12.07.413708

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